MICROSCOPE SYSTEM WITH TRANSILLUMINATION-BASED AUTOFOCUSING FOR PHOTOLUMINESCENCE IMAGING

Microscope system for, and methods of, imaging a sample including biological cells. In an exemplary method, light transmitted through the sample may be detected for a first set of focal positions to collect a first stack of images. Values of a focus metric may be calculated for the first stack of images. A candidate focal position may be determined based on the values. Photoluminescence may be detected from the sample for a second set of focal positions to collect a second stack of images. The second set of focal positions may define a smaller range than the first set of focal positions. At least one focal position of the second set of focal positions may be based on the candidate focal position. In other words, the candidate focal position may serve as a guide for finding a suitable photoluminescence focal position. 对包括生物细胞的样本进行成像的显微镜系统和方法。在示例性方法中，可针对第组焦点位置检测透射通过所述样本的光以收集第堆图像。可计算所述第堆图像的焦点度量的值。可基于所述值确定候选焦点位置。可针对第二组焦点位置检测来自所述样本的光致发光以收集第二堆图像。所述第二组焦点位置可比所述第组焦点位置限定的范围更小。所述第二组焦点位置中的至少个焦点位置可以是基于所述候选焦点位置的。换句话说，候选焦点位