Method for detecting prymnesium parvum

The invention provides a method for detecting prymnesium parvum. 6 LAMP primers are designed, namely P.P-FIP, P.P-BIP, P.P-LF, P.P-LB, P.P-F3 and P.P-B3, wherein the P.P-FIP is a 5' terminal biotin labeled primer; the P.P-LB is a 5' terminal thiocyanate fluorescein FITC labeled probe. The...

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Bibliographische Detailangaben
Hauptverfasser:	XU JILIN, YAN XIAOJUN, DANG CHENYANG, QIAO LONGLIANG, PANG JIANHU, HUANG HAILONG, ZHU PENG
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention provides a method for detecting prymnesium parvum. 6 LAMP primers are designed, namely P.P-FIP, P.P-BIP, P.P-LF, P.P-LB, P.P-F3 and P.P-B3, wherein the P.P-FIP is a 5' terminal biotin labeled primer; the P.P-LB is a 5' terminal thiocyanate fluorescein FITC labeled probe. The method comprises the following steps: preparing an LAMP reaction system containing three pairs of primers andsample templates; amplifying the LAMP reaction system, then detecting with an LFD test paper strip and subsequently performing sensitivity and specificity evaluation. The method provided by the invention is more practical and convenient that an existing method for detecting the prymnesium parvum by traditional technology PCR, has high sensitivity and specificity, can be applied to an actual field,is beneficial to detection and control on the outbreak of the prymnesium parvum and takes precautions in advance. When being applied to the detection on the harmful alga prymnesium parvum, the methodprovided by the invention