Method for improving in vitro fertilization ability of sex-controlled frozen semens of cow

The invention provides a purpose for improving in vitro fertilization ability of sex-controlled frozen semens by anthocyanin and pyrroloquinoline quinine, and provides semen washing liquid and insemination medium adding with the anthocyanin and the pyrroloquinoline quinine. The anthocyanin and the pyrroloquinoline quinine are added into the semen washing liquid and the insemination medium in the process of unfreezing and capacitation of the sex-controlled frozen semens of a cow to process the sex-controlled frozen semens of the cow, and then in vitro fertilization is performed. The results showed that the cleavage rate and blastocyst rate of the sex-controlled frozen semens of the cow are significantly increased and are significantly higher than the in vitro fertilization efficiency of unseparated frozen semens of a same bull, extensive application prospects are achieved, and popularization is worth. 本发明提出了花青素和吡咯喹啉醌在提高性控冻精体外受精能力方面的用途，并提出了添加花青素和吡咯喹啉醌的洗精液和受精液。本发明在牛性控冻精解冻、获能过程中，向洗精液和受精液中分别都加入花青素和吡咯喹啉醌，来处理牛性控冻精，而