PCR (polymerase chain reaction) Primer pair and method to detect Schlumbergera virus X and application of PCR primer pair

The invention discloses a PCR (polymerase chain reaction) primer pair and method to detect Schlumbergera virus X and application of the PCR primer pair. The primer pair includes an upstream primer SchVX CP-F: 5'-GGTCATCACCGGAAACTGT-3', and a downstream primer SchVX CP-R: 5'-CTTTGGGCTT...

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Bibliographische Detailangaben
Hauptverfasser:	BAI ZIQIN, WANG XIAOKE, ZHENG QIANMING, LI XINGZHONG, MA YUHUA, LIU TAO
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses a PCR (polymerase chain reaction) primer pair and method to detect Schlumbergera virus X and application of the PCR primer pair. The primer pair includes an upstream primer SchVX CP-F: 5'-GGTCATCACCGGAAACTGT-3', and a downstream primer SchVX CP-R: 5'-CTTTGGGCTTTCAGATCCTT-3'. The PCR primer pair can be applied to the detection on whether Hylocereus undulatus, Opuntia strictaand Schlumbergera truncata experience Schlumbergera virus X infection. 本发明公开了检测蟹爪兰X病毒所用PCR引物对、检测方法及其应用，该引物对包括上游引物SchVX CP-F：5'-GGTCATCACCGGAAACTGT-3'，下游引物SchVX CP-R：5'-CTTTGGGCTTTCAGATCCTT-3'。该PCR引物对能在检测火龙果、仙人掌及蟹爪兰是否存在蟹爪兰X病毒侵染中得到应用。