Porcine BVDV (Bovine Viral Diarrhea Virus) recombinant virus for expressing porcine epidemic diarrhea virus (PEDV) S genes and application
The invention discloses a porcine BVDV (Bovine Viral Diarrhea Virus) recombinant virus for expressing porcine epidemic diarrhea virus (PEDV) S genes and the application thereof. A neutralization antigenic epitope sequence of the PEDV S genes is inserted into a terminal N of a C nucleocapsid protein...
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| creator | SHI YING CHENG JINGHUA LI BENQIANG RAO BAIZHONG LIU HUILI TAO JIE |
| description | The invention discloses a porcine BVDV (Bovine Viral Diarrhea Virus) recombinant virus for expressing porcine epidemic diarrhea virus (PEDV) S genes and the application thereof. A neutralization antigenic epitope sequence of the PEDV S genes is inserted into a terminal N of a C nucleocapsid protein gene of porcine BVDV. A recombinant infectious cloning plasmid is obtained. The plasmid is linearized, subjected to in vitro transcription to obtain RNA and transfected with MDBK (Madin-Darby Bovine Kidney) cells, so that the porcine BVDV recombinant virus for expressing the PEDV S proteins can be successfully rescued. The porcine BVDV recombinant virus is capable of expressing a PEDV S foreign protein, and is simultaneously used for controlling the PEDV and BVDV. The porcine BVDV infectious clone provides suitable insertion sites for other exogenous genes, can serve as a recombinant viral vector, and provides an effective technology platform for researches such as subsequent in-vitro modification of RNA, insertion |
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A neutralization antigenic epitope sequence of the PEDV S genes is inserted into a terminal N of a C nucleocapsid protein gene of porcine BVDV. A recombinant infectious cloning plasmid is obtained. The plasmid is linearized, subjected to in vitro transcription to obtain RNA and transfected with MDBK (Madin-Darby Bovine Kidney) cells, so that the porcine BVDV recombinant virus for expressing the PEDV S proteins can be successfully rescued. The porcine BVDV recombinant virus is capable of expressing a PEDV S foreign protein, and is simultaneously used for controlling the PEDV and BVDV. 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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Porcine BVDV (Bovine Viral Diarrhea Virus) recombinant virus for expressing porcine epidemic diarrhea virus (PEDV) S genes and application |
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