Porcine BVDV (Bovine Viral Diarrhea Virus) recombinant virus for expressing porcine epidemic diarrhea virus (PEDV) S genes and application

The invention discloses a porcine BVDV (Bovine Viral Diarrhea Virus) recombinant virus for expressing porcine epidemic diarrhea virus (PEDV) S genes and the application thereof. A neutralization antigenic epitope sequence of the PEDV S genes is inserted into a terminal N of a C nucleocapsid protein gene of porcine BVDV. A recombinant infectious cloning plasmid is obtained. The plasmid is linearized, subjected to in vitro transcription to obtain RNA and transfected with MDBK (Madin-Darby Bovine Kidney) cells, so that the porcine BVDV recombinant virus for expressing the PEDV S proteins can be successfully rescued. The porcine BVDV recombinant virus is capable of expressing a PEDV S foreign protein, and is simultaneously used for controlling the PEDV and BVDV. The porcine BVDV infectious clone provides suitable insertion sites for other exogenous genes, can serve as a recombinant viral vector, and provides an effective technology platform for researches such as subsequent in-vitro modification of RNA, insertion