Biochip for unicellular positioning and screening based on micro nanosphere cavity array and preparation method of biochip

The invention relates to a biochip for unicellular positioning and screening based on a micro nanosphere cavity array and a preparation method of the biochip and belongs to the technical field of biochips. The biochip consists of an indium tin oxide substrate and an upper layer unit, wherein the upper layer unit is of a microsphere cavity bowl-shaped structure; the indium tin oxide substrate is transparent and has good conductivity; the upper layer unit of the microsphere cavity bowl-shaped structure is an independent isolation unit array formed by a plurality of micro nanosphere cavities; themicro nanosphere cavities are made of aluminum oxide; bottom substrates of the micro nanosphere cavities is exposed. The biochip is based on a microsphere cavity array biochip technique, the biochipis integrated with application of unicellular positioning and screening, operation difficulties of unicellular positioning and screening are effectively reduced, operation procedures are specified, and development of later det