PURIFICATION OF POLYMERASE COMPLEXES

Disclosed are methods for isolating polymerase complexes from a mixture of polymerase complex components. The polymerase complexes can comprise a nanopore to provide isolated nanopore sequencing complexes. The methods relate to the positive and negative isolation of the polymerase complexes and/or n...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Hauptverfasser:	DAVIS RANDALL W, FRANKLIN HELEN, CHECHELSKI JOHNSTON NATALIE B, AYER ARUNA, CRAIG TIMOTHY KELLOGG, CRISALLI PETER, CECH CYNTHIA, MABRY JOSHUA N, BIBILLO ARKADIUSZ, DHIMAN KIRTI
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
Online-Zugang:	Volltext bestellen
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	Disclosed are methods for isolating polymerase complexes from a mixture of polymerase complex components. The polymerase complexes can comprise a nanopore to provide isolated nanopore sequencing complexes. The methods relate to the positive and negative isolation of the polymerase complexes and/or nanopore sequencing complexes. Also disclosed is a nucleic acid adaptor for isolating active polymerase complexes, polymerase complexes comprising the nucleic acid adaptor, and methods for isolating active polymerase complexes using the nucleic acid adaptor. 公开了从聚合酶复合体组分的混合物分离聚合酶复合体的方法。聚合酶复合体可以包含纳米孔以提供分离的纳米孔测序复合体。所述方法涉及聚合酶复合体和/或纳米孔测序复合体的正和负分离。还公开了用于分离活性聚合酶复合体的核酸衔接子，包含核酸衔接子的聚合酶复合体，以及使用核酸衔接子分离活性聚合酶复合体的方法。