Fluorescent quantitative PCR detection method and detection kit of lactobacillus acidophilus component in lactobacillus beverage and application of detection kit

The invention discloses a fluorescent quantitative PCR detection method and a detection kit of a lactobacillus acidophilus component in lactobacillus beverage and an application of the detection kit.The fluorescent quantitative PCR detection method of the lactobacillus acidophilus component in lactobacillus beverage comprises the following steps: I, with DNA of a to-be-detected sample as a template, implementing fluorescent quantitative PCR amplification so as to obtain a PCR amplification product; II, detecting a fluorescence signal of the amplification product; and III, calculating the content of the lactobacillus acidophilus component in the to-be-detected sample, wherein a reaction system, which is applied to the PCR amplification, comprises a specific primer pair for amplifying the lactobacillus acidophilus component and a specific probe for the lactobacillus acidophilus component. According to the invention, primers and the probe are designed in accordance with an SPIDR conserved domain of the lactobaci