Culture medium for induced differentiation of pluripotent stem cells into hematopoietic stem cells and method thereof

The invention relates to a culture medium for induced differentiation of pluripotent stem cells into hematopoietic stem cells and a method thereof. The culture medium comprises a first-stage culture medium and a second-stage culture medium. According to the method, the first-stage culture medium and the second-stage culture medium are successively used for two-stage co-culture of pluripotent stemcells and OP9 cells. In comparison with the prior art, the invention has the following beneficial effects: by the use of the culture medium with the above two specific ingredients and concentrations and for induced differentiation of pluripotent stem cells into hematopoietic stem cells, induced differentiation of pluripotent stem cells is successively conducted, and the efficiency of differentiation of pluripotent stem cells into hematopoietic stem cells is raised. 本发明涉及种诱导多能干细胞分化成造血干细胞培养基及方法，所述培养基包括第阶段培养基和第二阶段培养基；所述方法包括依次使用第阶段培养基、第二阶段培养对多能干细胞与OP9细胞分两个阶段进行共培养。与现有技术相比，本发明具备如下有益效果：本发明通过采用上述两种特定成分和浓度的诱导多能干细胞分化成造血干细胞的培养基，