Method for improving in vitro fertilizable competence of vitrification freezing oocyte
The invention provides a method for improving in vitro fertilizable competence of vitrification freezing oocyte. The method disclosed by the invention comprises the following steps: performing combined treatment on bovine oocyte with a solution containing gadolinium and CLC before vitrification free...
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| creator | HAO HAISHENG PANG YUNWEI ZHAO YAHAN LIU YAN DU WEIHUA ZHAO XUEMING ZHU HUABIN |
| description | The invention provides a method for improving in vitro fertilizable competence of vitrification freezing oocyte. The method disclosed by the invention comprises the following steps: performing combined treatment on bovine oocyte with a solution containing gadolinium and CLC before vitrification freezing treatment, and performing vitrification freezing; treating with methyl-beta-cyclodextrin afterunfreezing, and performing in vitro fertilization. Results prove that the method is capable of obviously improving the cleavage rate and blastocyst rate of vitrification freezing bovine oocyte and achieving in vitro fertilization efficiency higher than that of fresh bovine oocyte, has a wide application prospect and is worthy of popularization.
本发明提供了种提高玻璃化冷冻牛卵母细胞体外受精能力的方法。本发明在玻璃化冷冻处理前，将牛卵母细胞采用含有钆和CLC的溶液联合处理，再进行玻璃化冷冻；解冻后采用甲基化-β-环糊精处理，而后进行体外受精。结果表明，该方法能够显著提高玻璃化冷冻牛卵母细胞的卵裂率和囊胚率，并且高于新鲜牛卵母细胞的体外受精效率，具有广泛的应用前景，值得推广。 |
| format | Patent |
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本发明提供了种提高玻璃化冷冻牛卵母细胞体外受精能力的方法。本发明在玻璃化冷冻处理前，将牛卵母细胞采用含有钆和CLC的溶液联合处理，再进行玻璃化冷冻；解冻后采用甲基化-β-环糊精处理，而后进行体外受精。结果表明，该方法能够显著提高玻璃化冷冻牛卵母细胞的卵裂率和囊胚率，并且高于新鲜牛卵母细胞的体外受精效率，具有广泛的应用前景，值得推广。</description><language>chi ; eng</language><subject>AGRICULTURE ; ANIMAL HUSBANDRY ; BEER ; BIOCHEMISTRY ; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; FISHING ; FORESTRY ; HUMAN NECESSITIES ; HUNTING ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PEST REPELLANTS OR ATTRACTANTS ; PLANT GROWTH REGULATORS ; PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; TRAPPING ; VINEGAR ; WINE</subject><creationdate>2018</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20180928&DB=EPODOC&CC=CN&NR=108588011A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20180928&DB=EPODOC&CC=CN&NR=108588011A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>HAO HAISHENG</creatorcontrib><creatorcontrib>PANG YUNWEI</creatorcontrib><creatorcontrib>ZHAO YAHAN</creatorcontrib><creatorcontrib>LIU YAN</creatorcontrib><creatorcontrib>DU WEIHUA</creatorcontrib><creatorcontrib>ZHAO XUEMING</creatorcontrib><creatorcontrib>ZHU HUABIN</creatorcontrib><title>Method for improving in vitro fertilizable competence of vitrification freezing oocyte</title><description>The invention provides a method for improving in vitro fertilizable competence of vitrification freezing oocyte. The method disclosed by the invention comprises the following steps: performing combined treatment on bovine oocyte with a solution containing gadolinium and CLC before vitrification freezing treatment, and performing vitrification freezing; treating with methyl-beta-cyclodextrin afterunfreezing, and performing in vitro fertilization. Results prove that the method is capable of obviously improving the cleavage rate and blastocyst rate of vitrification freezing bovine oocyte and achieving in vitro fertilization efficiency higher than that of fresh bovine oocyte, has a wide application prospect and is worthy of popularization.
本发明提供了种提高玻璃化冷冻牛卵母细胞体外受精能力的方法。本发明在玻璃化冷冻处理前，将牛卵母细胞采用含有钆和CLC的溶液联合处理，再进行玻璃化冷冻；解冻后采用甲基化-β-环糊精处理，而后进行体外受精。结果表明，该方法能够显著提高玻璃化冷冻牛卵母细胞的卵裂率和囊胚率，并且高于新鲜牛卵母细胞的体外受精效率，具有广泛的应用前景，值得推广。</description><subject>AGRICULTURE</subject><subject>ANIMAL HUSBANDRY</subject><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>FISHING</subject><subject>FORESTRY</subject><subject>HUMAN NECESSITIES</subject><subject>HUNTING</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PEST REPELLANTS OR ATTRACTANTS</subject><subject>PLANT GROWTH REGULATORS</subject><subject>PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>TRAPPING</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2018</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNy0EKwjAQRuFuXIh6h_EAQoMI2UpR3OhK3JYY_9iBNBPSoWBPL4oHcPU235tXtzO0kwcFKcR9LjJyehInGlmLUEBRjjy5ewR56TMUyYMkfAEH9k5ZEoUCTJ9VxL8Uy2oWXByw-nVRrY-Ha3PaIEuLITuPBG2bi6ntztramP32H_MGgYc6aA</recordid><startdate>20180928</startdate><enddate>20180928</enddate><creator>HAO HAISHENG</creator><creator>PANG YUNWEI</creator><creator>ZHAO YAHAN</creator><creator>LIU YAN</creator><creator>DU WEIHUA</creator><creator>ZHAO XUEMING</creator><creator>ZHU HUABIN</creator><scope>EVB</scope></search><sort><creationdate>20180928</creationdate><title>Method for improving in vitro fertilizable competence of vitrification freezing oocyte</title><author>HAO HAISHENG ; PANG YUNWEI ; ZHAO YAHAN ; LIU YAN ; DU WEIHUA ; ZHAO XUEMING ; ZHU HUABIN</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN108588011A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2018</creationdate><topic>AGRICULTURE</topic><topic>ANIMAL HUSBANDRY</topic><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>FISHING</topic><topic>FORESTRY</topic><topic>HUMAN NECESSITIES</topic><topic>HUNTING</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PEST REPELLANTS OR ATTRACTANTS</topic><topic>PLANT GROWTH REGULATORS</topic><topic>PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>TRAPPING</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>HAO HAISHENG</creatorcontrib><creatorcontrib>PANG YUNWEI</creatorcontrib><creatorcontrib>ZHAO YAHAN</creatorcontrib><creatorcontrib>LIU YAN</creatorcontrib><creatorcontrib>DU WEIHUA</creatorcontrib><creatorcontrib>ZHAO XUEMING</creatorcontrib><creatorcontrib>ZHU HUABIN</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>HAO HAISHENG</au><au>PANG YUNWEI</au><au>ZHAO YAHAN</au><au>LIU YAN</au><au>DU WEIHUA</au><au>ZHAO XUEMING</au><au>ZHU HUABIN</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Method for improving in vitro fertilizable competence of vitrification freezing oocyte</title><date>2018-09-28</date><risdate>2018</risdate><abstract>The invention provides a method for improving in vitro fertilizable competence of vitrification freezing oocyte. The method disclosed by the invention comprises the following steps: performing combined treatment on bovine oocyte with a solution containing gadolinium and CLC before vitrification freezing treatment, and performing vitrification freezing; treating with methyl-beta-cyclodextrin afterunfreezing, and performing in vitro fertilization. Results prove that the method is capable of obviously improving the cleavage rate and blastocyst rate of vitrification freezing bovine oocyte and achieving in vitro fertilization efficiency higher than that of fresh bovine oocyte, has a wide application prospect and is worthy of popularization.
本发明提供了种提高玻璃化冷冻牛卵母细胞体外受精能力的方法。本发明在玻璃化冷冻处理前，将牛卵母细胞采用含有钆和CLC的溶液联合处理，再进行玻璃化冷冻；解冻后采用甲基化-β-环糊精处理，而后进行体外受精。结果表明，该方法能够显著提高玻璃化冷冻牛卵母细胞的卵裂率和囊胚率，并且高于新鲜牛卵母细胞的体外受精效率，具有广泛的应用前景，值得推广。</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | AGRICULTURE ANIMAL HUSBANDRY BEER BIOCHEMISTRY BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FISHING FORESTRY HUMAN NECESSITIES HUNTING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PEST REPELLANTS OR ATTRACTANTS PLANT GROWTH REGULATORS PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TRAPPING VINEGAR WINE |
| title | Method for improving in vitro fertilizable competence of vitrification freezing oocyte |
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