Method for improving in vitro fertilizable competence of vitrification freezing oocyte

The invention provides a method for improving in vitro fertilizable competence of vitrification freezing oocyte. The method disclosed by the invention comprises the following steps: performing combined treatment on bovine oocyte with a solution containing gadolinium and CLC before vitrification freezing treatment, and performing vitrification freezing; treating with methyl-beta-cyclodextrin afterunfreezing, and performing in vitro fertilization. Results prove that the method is capable of obviously improving the cleavage rate and blastocyst rate of vitrification freezing bovine oocyte and achieving in vitro fertilization efficiency higher than that of fresh bovine oocyte, has a wide application prospect and is worthy of popularization. 本发明提供了种提高玻璃化冷冻牛卵母细胞体外受精能力的方法。本发明在玻璃化冷冻处理前，将牛卵母细胞采用含有钆和CLC的溶液联合处理，再进行玻璃化冷冻；解冻后采用甲基化-β-环糊精处理，而后进行体外受精。结果表明，该方法能够显著提高玻璃化冷冻牛卵母细胞的卵裂率和囊胚率，并且高于新鲜牛卵母细胞的体外受精效率，具有广泛的应用前景，值得推广。