Quantitative detection kit for human vWF (von Willebrand Factor)

The invention relates to a quantitative detection kit for a human vWF (von Willebrand Factor). A quantitative range of the quantitative detection kit is 3.2 to 51.5 mIU/ml; the quantitative detectionkit comprises (1) a microtitration plate coated by human type-III collagen and (2) a human anti-vWF d...

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Hauptverfasser: DENG ZHIJUN, YUE SHENGLAN, PENG YAN, CHEN KEJIN, HU YONG, WANG QIAN, WANG FEIFEI, LIN LIANZHEN, LI CESHENG, LI TAOJING, ZHOU ZHIJUN, LI JUAN, ZHAN QIAN, TAN MIN
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention relates to a quantitative detection kit for a human vWF (von Willebrand Factor). A quantitative range of the quantitative detection kit is 3.2 to 51.5 mIU/ml; the quantitative detectionkit comprises (1) a microtitration plate coated by human type-III collagen and (2) a human anti-vWF detecting antibody indicating solution labeled by HRP (Horse Reddish Peroxidase). A preparation method of the microtitration plate coated by the human type-III collagen comprises the following steps: taking the microtitration plate as a carrier, diluting the human type-III collagen to 20 mug/ml by using a coating buffer solution, coating the microtitration plate according to the amount of 100 mul per pore, coating at 2 to 8 DEG C, and staying overnight. A preparation method of the human anti-vWFdetecting antibody indicating solution labeled by the HRP comprises the step: diluting a human anti-vWF detecting antibody to 0.5 mug/ml by using a detecting antibody dilution solution. 本发明涉及种人血管性血友病因子(vWF)活性的定量检测试剂盒,所述检测试剂盒的