Method for detecting antibody-producing cells by using in-vitro quantitative hemolysis spectrophotometry

The invention discloses a method for detecting antibody-producing cells by using an in-vitro quantitative hemolysis spectrophotometry. The method comprises the following steps: (1) taking a cell culture plate, and adding porcine spleen lymphocyte suspension and physiological saline into each well of a blank group; adding the porcine spleen lymphocyte suspension, the physiological saline and sheepred blood cell (SRBC) suspension into each well of a test group, and culturing for 72-84h; (2) adding the sensitized SRBC suspension into each well, continuously culturing for 1-2h, centrifuging, taking 500 volume parts of supernatant from each well and placing into another cell culture plate, using a microplate reader to determine the absorbance of the supernatant in each well at the position of415 nm wavelength, and recording a result as OD415nm; (3) expressing experimental results by means of an antibody production cell positive rate A, wherein A=[(test group OD415nm mean value/blank groupOD415nm mean value)-1]*100