Method for detecting viable bacteria in staphylococcus aureus

The invention provides a method for detecting viable bacteria in staphylococcus aureus in order to solve the problems of false positive and false negative when a PMA (Propidium Monoazide)-qPCR (quantitative Polymerase Chain Reaction) method is adopted for detecting the viable bacteria in the prior art. According to the method provided by the invention, an SDS (Sodium Dodecyl Sulfonate) treatment step is added before PMA treatment on the basis of the PMA-qPCR method, a lysostaphin treatment step is added before DNA (Deoxyribonucleic Acid) extracting, and bacillus cereus DNA is added as an amplification internal reference during a qPCR process, so that the problems of false positive and false negative in an original method are well solved, and the sensitivity and the specificity of the detecting method are improved. 为解决现有技术中采用PMA-qPCR方法检测活菌时的假阳性和假阴性问题，本发明提供了种改进的检测和确定乳中金黄色葡萄球菌活菌的方法，其在PMA-qPCR方法的基础上，在PMA处理前增加了SDS处理步骤，在提取DNA前，增加了溶葡萄球菌素处理步骤，在qPCR过程中增加了蜡样芽孢杆菌DNA作为扩增内参，很好的解决了原有方法的假阳性和假阴性的问题，提高了检测方法的灵敏性和特异性。