Kit for construction of single-cell transcriptome sequencing library, and library construction method

The invention provides a construction method for a single-cell transcriptome sequencing library. The construction method comprises the following steps: step A, amplifying a single-cell transcriptome to construct a single-cell cDNA library; and step B, subjecting the cDNA library to fragmentation library construction so as to obtain the single-cell transcriptome sequencing library. According to theinvention, ingenious improvement is made on the basis of traditional single-cell transcriptome sequencing library construction methods so as to significantly improve the yield and purity of the single-cell sequencing library, and thus, the stability of data output is ensured. 本申请人提供了种单细胞转录组测序文库的构建方法，包括以下步骤：步骤A：对单细胞转录组进行扩增，构建单细胞cDNA文库；步骤B：对cDNA文库进行片段化文库的构建，从而得到单细胞转录组测序文库。本发明在传统的单细胞转录组测序文库构建方法的基础上进行了巧妙的改进，以显著提高单细胞测序文库的产量及纯度，从而保证数据产出的稳定性。