Engineering strain wp4-C23O for degrading polycyclic aromatic hydrocarbons (PAHs) as well as construction method and application of engineering strain wp4-C23O

The invention discloses an engineering strain wp4-C23O for degrading polycyclic aromatic hydrocarbons (PAHs) as well as a construction method and application of the engineering strain wp4-C23O. The engineering strain wp4-C23O is obtained by carrying out polymerase chain reaction (PCR) amplification on a catechol-2, 3-dioxygenase (C23O) gene of pseudomonas sp. wp3-1 and ligating to a suicide vectorpUTmini-Tn5 to obtain a recombinant vector pUTmini-Tn5-C23O, and integrating the C23O gene in the recombinant vector pUTmini-Tn5-C23O into the chromosomal DNA of pseudomonas stutzeri wp4 by means ofmini-Tn5 transposition under the action of triparetal conjugation. Compared with a strain wp4, the engineering strain wp4-C23O has a remarkably improved degradation rate of pyrene; the degradation rate is increased by 11.45%, and the problem of genetic instability is solved. 本发明公开种多环芳烃降解工程菌wp4-C23O及其构建方法和应用，工程菌wp4-C23O由PCR扩增克隆假单胞菌（. wp3-1）的邻苯二酚-2，3-双加氧酶（C23O）基因，连接于自杀性载体pUTmini-Tn5得到重组载体pUTmini-Tn5-C23O，通过三亲接合作用经mini-Tn5转座将