Method for measuring potency of chicken bursin by using in vitro quantitative hemolysis spectrophotometry

The invention discloses a method for measuring the potency of chicken bursin by using the in vitro quantitative hemolysis spectrophotometry. The method comprises the following steps: firstly, preparing porcine splenic lymphocyte suspension under a germfree condition, adding SRBC (Sheep Red Blood Cell) and a complete medium, placing the medium at 37 DEG C for cultivation or sensitization, adding the SRBC and an addiment after certain time, placing the medium at 37 DEG C for incubation, taking supernate, putting the supernate into a microplate reader to measure an OD value, and calculating the activity of the bursin through a formula. The method disclosed by the invention has the advantages of sensitivity, stability, objectiveness and the like, and is easy and convenient to operate, short inperiod and low in cost. 本发明公开了利用体外定量溶血分光光度法测定鸡法氏囊素效力的方法，首先无菌制备猪脾脏淋巴细胞悬液，加入SRBC及完全培养基放置37℃培养进行致敏，定时间后再加入SRBC和补体，放置37℃孵育，取上清放置酶标仪测定OD值，然后通过公式计算法氏囊素活力。本发明方法具有灵敏、稳定、客观等优点，并且操作简便，周期短，成本低。