Saccharomyces cerevisiae capable of producing ethyl lactate and construction method and application of saccharomyces cerevisiae
The invention belongs to the technical field of bioengineering, relates to the breeding of industrial microorganisms, and particularly relates to a method for increasing the yield of ethyl lactate ofsaccharomyces cerevisiae and an application of the method. The lactic dehydrogenase gene ldhL1 is ove...
Gespeichert in:
| Hauptverfasser: | , , , , , |
|---|---|
| Format: | Patent |
| Sprache: | chi ; eng |
| Schlagworte: | |
| Online-Zugang: | Volltext bestellen |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | |
| container_start_page | |
| container_title | |
| container_volume | |
| creator | REN JINYING CHEN YEFU LIU GANG LI JIE XIAO DONGGUANG GUO XUEWU |
| description | The invention belongs to the technical field of bioengineering, relates to the breeding of industrial microorganisms, and particularly relates to a method for increasing the yield of ethyl lactate ofsaccharomyces cerevisiae and an application of the method. The lactic dehydrogenase gene ldhL1 is overexpressed in an original strain to obtain a yeast strain having a certain capability of producingL-lactate and ethyl lactate, compared with the condition that the original strain is taken as the producing strain, the yield of the produced ethyl lactate of the strain is increased by more than 40%after the ldhL1 is added as an external source to catalyze to produce equal amount of lactate, the unexpected technical effect is achieved, the isoamyl alcohol is reduced by about 57.7% in comparisonwith that of the original strain, the phenethyl alcohol is reduced by about 52.11%; meanwhile, the PGK1P is chosen to overexpress the Candida antarctica lipase B, and the yield of the ethyl lactate isup to 210.25 mg/L. The metho |
| format | Patent |
| fullrecord | <record><control><sourceid>epo_EVB</sourceid><recordid>TN_cdi_epo_espacenet_CN108486176A</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>CN108486176A</sourcerecordid><originalsourceid>FETCH-epo_espacenet_CN108486176A3</originalsourceid><addsrcrecordid>eNqNjbEKwkAQRNNYiPoP6wcIBiWmlaBY2Wgf1r2NObjcLncXIZW_bgi2gtXAm8fMPHvfkKjFIN1AHIE48MtGiwyEig_HIA1oENOT9U_g1A4OHFLCxIDeAImPKfSUrHjoxl7MxFHVWcIJjxPxx80ymzXoIq--ucjW59O9umxYpeaoSOw51dU135b7ssgPxXH3j_MBhXlKCQ</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>patent</recordtype></control><display><type>patent</type><title>Saccharomyces cerevisiae capable of producing ethyl lactate and construction method and application of saccharomyces cerevisiae</title><source>esp@cenet</source><creator>REN JINYING ; CHEN YEFU ; LIU GANG ; LI JIE ; XIAO DONGGUANG ; GUO XUEWU</creator><creatorcontrib>REN JINYING ; CHEN YEFU ; LIU GANG ; LI JIE ; XIAO DONGGUANG ; GUO XUEWU</creatorcontrib><description>The invention belongs to the technical field of bioengineering, relates to the breeding of industrial microorganisms, and particularly relates to a method for increasing the yield of ethyl lactate ofsaccharomyces cerevisiae and an application of the method. The lactic dehydrogenase gene ldhL1 is overexpressed in an original strain to obtain a yeast strain having a certain capability of producingL-lactate and ethyl lactate, compared with the condition that the original strain is taken as the producing strain, the yield of the produced ethyl lactate of the strain is increased by more than 40%after the ldhL1 is added as an external source to catalyze to produce equal amount of lactate, the unexpected technical effect is achieved, the isoamyl alcohol is reduced by about 57.7% in comparisonwith that of the original strain, the phenethyl alcohol is reduced by about 52.11%; meanwhile, the PGK1P is chosen to overexpress the Candida antarctica lipase B, and the yield of the ethyl lactate isup to 210.25 mg/L. The metho</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROCESSES USING MICROORGANISMS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2018</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20180904&DB=EPODOC&CC=CN&NR=108486176A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,777,882,25545,76296</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20180904&DB=EPODOC&CC=CN&NR=108486176A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>REN JINYING</creatorcontrib><creatorcontrib>CHEN YEFU</creatorcontrib><creatorcontrib>LIU GANG</creatorcontrib><creatorcontrib>LI JIE</creatorcontrib><creatorcontrib>XIAO DONGGUANG</creatorcontrib><creatorcontrib>GUO XUEWU</creatorcontrib><title>Saccharomyces cerevisiae capable of producing ethyl lactate and construction method and application of saccharomyces cerevisiae</title><description>The invention belongs to the technical field of bioengineering, relates to the breeding of industrial microorganisms, and particularly relates to a method for increasing the yield of ethyl lactate ofsaccharomyces cerevisiae and an application of the method. The lactic dehydrogenase gene ldhL1 is overexpressed in an original strain to obtain a yeast strain having a certain capability of producingL-lactate and ethyl lactate, compared with the condition that the original strain is taken as the producing strain, the yield of the produced ethyl lactate of the strain is increased by more than 40%after the ldhL1 is added as an external source to catalyze to produce equal amount of lactate, the unexpected technical effect is achieved, the isoamyl alcohol is reduced by about 57.7% in comparisonwith that of the original strain, the phenethyl alcohol is reduced by about 52.11%; meanwhile, the PGK1P is chosen to overexpress the Candida antarctica lipase B, and the yield of the ethyl lactate isup to 210.25 mg/L. The metho</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROCESSES USING MICROORGANISMS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2018</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNjbEKwkAQRNNYiPoP6wcIBiWmlaBY2Wgf1r2NObjcLncXIZW_bgi2gtXAm8fMPHvfkKjFIN1AHIE48MtGiwyEig_HIA1oENOT9U_g1A4OHFLCxIDeAImPKfSUrHjoxl7MxFHVWcIJjxPxx80ymzXoIq--ucjW59O9umxYpeaoSOw51dU135b7ssgPxXH3j_MBhXlKCQ</recordid><startdate>20180904</startdate><enddate>20180904</enddate><creator>REN JINYING</creator><creator>CHEN YEFU</creator><creator>LIU GANG</creator><creator>LI JIE</creator><creator>XIAO DONGGUANG</creator><creator>GUO XUEWU</creator><scope>EVB</scope></search><sort><creationdate>20180904</creationdate><title>Saccharomyces cerevisiae capable of producing ethyl lactate and construction method and application of saccharomyces cerevisiae</title><author>REN JINYING ; CHEN YEFU ; LIU GANG ; LI JIE ; XIAO DONGGUANG ; GUO XUEWU</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN108486176A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2018</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROCESSES USING MICROORGANISMS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>REN JINYING</creatorcontrib><creatorcontrib>CHEN YEFU</creatorcontrib><creatorcontrib>LIU GANG</creatorcontrib><creatorcontrib>LI JIE</creatorcontrib><creatorcontrib>XIAO DONGGUANG</creatorcontrib><creatorcontrib>GUO XUEWU</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>REN JINYING</au><au>CHEN YEFU</au><au>LIU GANG</au><au>LI JIE</au><au>XIAO DONGGUANG</au><au>GUO XUEWU</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Saccharomyces cerevisiae capable of producing ethyl lactate and construction method and application of saccharomyces cerevisiae</title><date>2018-09-04</date><risdate>2018</risdate><abstract>The invention belongs to the technical field of bioengineering, relates to the breeding of industrial microorganisms, and particularly relates to a method for increasing the yield of ethyl lactate ofsaccharomyces cerevisiae and an application of the method. The lactic dehydrogenase gene ldhL1 is overexpressed in an original strain to obtain a yeast strain having a certain capability of producingL-lactate and ethyl lactate, compared with the condition that the original strain is taken as the producing strain, the yield of the produced ethyl lactate of the strain is increased by more than 40%after the ldhL1 is added as an external source to catalyze to produce equal amount of lactate, the unexpected technical effect is achieved, the isoamyl alcohol is reduced by about 57.7% in comparisonwith that of the original strain, the phenethyl alcohol is reduced by about 52.11%; meanwhile, the PGK1P is chosen to overexpress the Candida antarctica lipase B, and the yield of the ethyl lactate isup to 210.25 mg/L. The metho</abstract><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext_linktorsrc |
| identifier | |
| ispartof | |
| issn | |
| language | chi ; eng |
| recordid | cdi_epo_espacenet_CN108486176A |
| source | esp@cenet |
| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Saccharomyces cerevisiae capable of producing ethyl lactate and construction method and application of saccharomyces cerevisiae |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-20T19%3A47%3A50IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-epo_EVB&rft_val_fmt=info:ofi/fmt:kev:mtx:patent&rft.genre=patent&rft.au=REN%20JINYING&rft.date=2018-09-04&rft_id=info:doi/&rft_dat=%3Cepo_EVB%3ECN108486176A%3C/epo_EVB%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true |