In vitro preservation method of plasmopara viticola

The invention discloses an in vitro preservation method of plasmopara viticola. According to the method, plasmopara viticola is embedded in agar powder, and then preserved at -80 DEG C; after 90 daysof preservation, healthy leaves of grapes are still pathogenic for back grafting; and a specific preservation treatment method and conditions of plasmopara viticola, and the processes of determinationof sporangium pathogenicity after preservation are also included in the method. The method provided by the invention is an ideal, simple and effective preservation method. 本发明公开种霜霉病菌离体保存方法，是将葡萄霜霉病菌包埋于琼脂粉中，然后在-80℃条件下进行保存，保存90d后回接葡萄健康叶片仍有致病性，还包括葡萄霜霉病菌的具体的保存处理方法及条件、保存后孢子囊致病性的测定等过程。本发明的方法是种理想的、简单有效的保存方法。