Indomethacin quantum dot immunochromatographic detection card based on signal amplification system and detection method

The invention relates to an indomethacin quantum dot immunochromatographic detection card based on a signal amplification system and a detection method. The detection card comprises a liner plate, a detection antigen release pad, a quantum dot probe release pad, a chromatographic membrane and a wate...

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Bibliographische Detailangaben
Hauptverfasser: HUANG WANQIONG, ZHANG PENG, ZHU ZHE, LIN XIUYU, GUO JIEBIAO, WU LANJIE
Format: Patent
Sprache:chi ; eng
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Beschreibung
Zusammenfassung:The invention relates to an indomethacin quantum dot immunochromatographic detection card based on a signal amplification system and a detection method. The detection card comprises a liner plate, a detection antigen release pad, a quantum dot probe release pad, a chromatographic membrane and a water absorbent pad; the detection antigen release pad comprises a detection antigen coupled by ovalbumin and indomethacin; the quantum dot probe release pad comprises a quantum dot probe labeled with an anti-ovalbumin antibody; the chromatographic membrane is provided with a detection line and a quality control line, the detection line fixes an anti-indomethacin antibody, and the quality control line fixes an ovalbumin antigen. The detection antigen is dissolved from the release pad into a detection system to form a ternary system immune complex with a detection antibody and a labeled antibody so as to generate a fluorescence detection signal. Under the detection and reference functions of thequality control line, whe