Method for degrading aflatoxin B1 in peanut meal

The invention relates to a method for degrading aflatoxin B1 in peanut meal. The method comprises the following steps: culturing preserved bacillus subtilis into bacillus subtilis bacterium liquid; preparing an LB fluid medium comprising 10g of peptone, 10g of sodium chloride and 5g of yeast extract...

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Bibliographische Detailangaben
Hauptverfasser:	LEI YUANPEI, WANG XINYUE, WANG LI, LI PING, PAN GUIXIA, ZHENG WENGE, ZHENG RUI, JIAO JIANGTAO
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FOODS OR FOODSTUFFS FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BYSUBCLASSES A23B - A23J HUMAN NECESSITIES METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OFNUTRITIVE QUALITIES, PHYSICAL TREATMENT THEIR TREATMENT, NOT COVERED BY OTHER CLASSES VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention relates to a method for degrading aflatoxin B1 in peanut meal. The method comprises the following steps: culturing preserved bacillus subtilis into bacillus subtilis bacterium liquid; preparing an LB fluid medium comprising 10g of peptone, 10g of sodium chloride and 5g of yeast extract powder per liter, regulating the pH value to be 7.2, split charging 50mL of medium into a 250ml oftriangular flask, and sealing by 16-layer gauze and 4-layer newspaper; sterilizing in a high temperature sterilizing oven for 20 minutes, inoculating the preserved bacillus subtilis into the sterilized medium, culturing at 37 DEG C and 200r for 24 hours for later use. Therefore, the method disclosed by the invention has the advantages of being wide in application range, capable of effectively degrading the aflatoxin in the peanut meal and convenient to operate. 本发明创造涉及种降解花生粕中的黄曲霉毒素B1的方法，它包括将保存的枯草芽孢杆菌培养成枯草芽孢杆菌菌液：配制LB液体培养基：（每升）为蛋白胨10 g，氯化钠10 g，酵母浸粉5 g，pH值调整到7.2，将50 mL培养基分装至250 ml三角瓶中，使用16层纱布加4层报纸封口，然后在高压灭菌锅中灭菌20min，将保存的