Method for screening MSAP (Methylation-sensitive Amplified Polymorphism) site related with thorns of lycium ruthenicum

The invention discloses a method for screening an MSAP (Methylation-sensitive Amplified Polymorphism) site related with thorns of lycium ruthenicum. The method comprises the following steps: 1) obtaining clonal tissue culture plants with a consistent genetic background and transplanting; 2) obtaining thorn or thorn-free type lycium ruthenicum with the consistent genetic background through controlling water/nutrients of potted plants; 3) extracting total DNA (Deoxyribonucleic Acid) of thorn and thorn-free lycium ruthenicum leaves; 4) carrying out dual enzyme digestion on the extracted total DNAand connecting a connector; 5) carrying out PCR (Polymerase Chain Reaction) pre-amplification on an enzyme digestion connecting product; 6) carrying out selective PCR amplification on a pre-amplifiedproduct; 7) carrying out capillary electrophoresis on a selective PCR amplification product; 8) screening a specific MSAP site of the thorn-free leaves; 9) recycling, sequencing and analyzing a specific band. According to the