Pseudorabies/porcine parvovirus infection combined inactivate vaccine and suspension culture preparation method

The invention belongs to the technical field of veterinary biological products and particularly relates to a pseudorabies/porcine parvovirus infection combined inactivate vaccine and a suspension culture preparation method. The preparation method comprises the following steps: preparing a pseudorabi...

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Hauptverfasser: ZHANG JIE, ZHOU MINGGUANG, CAO YI, JIN JIANYUN, YIN ZHENGYAN, SU SHUNPAN, SU XIUCHAN, FANG YULIN, HUANG HUIJUN, CHEN BIN, ZHONG EN, HONG DENG, XU GAOYUAN, CHEN ZHANGBIAO, CHEN GUANPING
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention belongs to the technical field of veterinary biological products and particularly relates to a pseudorabies/porcine parvovirus infection combined inactivate vaccine and a suspension culture preparation method. The preparation method comprises the following steps: preparing a pseudorabies virus suspension culture antigen and a porcine parvovirus infection virus suspension culture antigen; proportionally mixing the inactivated pseudorabies virus and porcine parvovirus infection virus antigen solutions, and then adding an adjuvant to fully emulsify to obtain the pseudorabies/porcineparvovirus infection combined inactivate vaccine. The suspension culture processes of the pseudorabies virus antigen solution and the porcine parvovirus infection virus antigen solution are established, the virus antigen solutions prepared through the suspension culture process have the advantages of high antigen content, large batch of the antigen and stable batch, the preparation method greatlyreduces the use of manpow