Method of utilizing RNA nucleic acid aptamer and nanogold to detect theophylline in serum

The invention discloses a method of utilizing RNA nucleic acid aptamer and nanogold to detect theophylline in serum. The method includes following steps: pretreating biological and clinical samples, hybridizing an embedment probe and target miRNA, connecting a capture sequence onto a surface modified ELISA plate, performing nucleic acid hybridization to form an ELISA plate-embedment probe-miRNA compound, checking through RNase ONE ribonuclease, and using a chemiluminescence method to detect target miRNA content and theophylline content. According to a pretreatment scheme in Direct PCR technology, biological cells or clinical lung cancer samples are chemically and biologically treated to degrade protein and DNA ingredients and to release total RNA therein without RNA extraction, so that experimental process is simplified, detection time is shortened, and precious clinical tumor samples can be saved. 本发明公开了种利用RNA核酸适配体及纳米金检测血清中的茶碱的方法，包括如下步骤：生物及临床样本的前处理、嵌合探针与目标miRNA杂交、连接捕捉序列于表面改性酶标板上、核酸杂交形成酶标板-嵌合探针-miRNA复合物、RNase