Preparation method and application of chitosan immobilized zymohexase with high acetaldehyde tolerance

The invention discloses a preparation method of chitosan immobilized zymohexase with high acetaldehyde tolerance. The preparation method comprises the following steps: 1) dissolving a certain amount of chitosan in an acetic acid solution to form a colloidal solution, and dropwise adding the colloidal solution in a coagulating agent (1 mol/L of NaOH) to form spherical particles; and 2) immobilizingpurified and desalted 2-deoxy-d-ribosyl-5-phosphate aldolase and a chitosan carrier prepared in step 1) in water bath at the room temperature. The chitosan immobilized zymohexase serves as a catalystto catalyze 2-deoxy-D-ribosyl-5-phosphoric acid to obtain 3-phosphoglyceraldehyde and acetaldehyde, tolerance to the substrate acetaldehyde is improved remarkably, and meanwhile, the recycling operation to enzyme is simplified. 本发明公开了种高乙醛耐受性的壳聚糖固定化醛缩酶的制备方法：包括以下步骤：1)取定量的壳聚糖溶乙酸溶液中，形成胶状溶液，滴加到凝结剂(1mol/L的NaOH)中形成球形颗粒，2)将纯化脱盐后的2-脱氧-d-核糖-5-磷酸醛缩酶与1)中制备的壳聚糖载体在室温下水浴中进行固定。采用该壳聚糖固定化醛缩酶为催化剂催化2-脱氧-D-核糖-5-磷酸，得到3-磷酸甘油醛和乙醛，对底物乙醛的耐受性显著提高，同