High-throughout sequencing method of genes relative to neurological and psychiatric drugs and application of high-throughout sequencing method

The invention belongs to the field of neurological and psychiatric gene detection and particularly relates to a high-throughout sequencing method of genes relative to neurological and psychiatric drugs and application of the high-throughout sequencing method. The invention provides the high-througho...

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Hauptverfasser: ZHONG SHILONG, YAO QICONG, DENG MEIYING, LU MANYUN, ZHOU HUANGKAI, XU YUXUAN
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention belongs to the field of neurological and psychiatric gene detection and particularly relates to a high-throughout sequencing method of genes relative to neurological and psychiatric drugs and application of the high-throughout sequencing method. The invention provides the high-throughout sequencing method of the genes relative to the neurological and psychiatric drugs and the application of the high-throughout sequencing method. The method comprises the following steps of acquisition of a genome DNA, target gene multiplex PCR amplification reaction and product purification, linker sequence PCR reaction and product purification, library detection, high-throughout sequencing and the like. The method can be used for detecting 29 SNP loci of 20 genes relative to drugs for neurological and psychiatric diseases at one time, can cover a vast majority of first-line and second-line drugs for resisting schizophrenia, depression, anxiety, epilepsy, Parkinson's disease, hyperkineticdisorder, pains and the l