General molecular marker for detecting strong blast resistance multiple alleles Pi-2/gm/zt and application

The invention discloses a general molecular marker for detecting strong blast resistance multiple alleles Pi-2/gm/zt and application. The invention further discloses a method for detecting the genes.The method comprises the following steps of extracting a rice sample genome DNA to be detected; utilizing a primer pair for performing PCR amplification; detecting whether a resistance gene donor parent and a receptor parent needing to be improved have polymorphism or not, if the polymorphism exists, judging whether a rice sample to be detected contains strong blast resistance genes Pi-2 or Pi-gmor Pi-zt genes or not according to a PCR amplification product. The sequence of the primer pair is shown in SEQ ID NO.1 and SEQ ID NO.2. The molecule assistant selection efficiency of a new strong blast resistance rice variety can be improved. 本发明公开了种检测抗稻瘟病复等位基因Pi-2/gm/zt的通用分子标记及应用。本发明还公开了种检测上述基因的方法，包括以下步骤：提取待检测水稻样品基因组DNA；利用引物对进行PCR扩增；检测抗性基因供体亲本和需要改良材受体亲本是否存在多态性，如果存在多态性，根据PCR扩增产物判断待测水稻样品是否含有抗稻瘟病基因Pi-2或Pi-gm或Pi-zt基因。所述的引物对序