Preparation method of temperature-sensitive kidney extracellular matrix (ECM) hydrogel

The invention discloses a preparation method of temperature-sensitive kidney extracellular matrix (ECM) hydrogel. The method concretely comprises the following steps: carrying out primary treatment onthe kidney, carrying out decellularized perfusion on the kidney, washing the kidney, dehydrating and sterilizing, stirring for digesting, gelatinizing. The method utilizes a perfusion method to perform rinsing and decellularization by means of renal arterial intubation; according to the distribution of the blood vessels, the decellularized liquid can reach almost every kidney tissue, so that thedecellularization is more fully performed. Furthermore, the components obtained after cell rupture can flow along with liquid and is discharged in time, so that the decellularization efficiency is higher, and the possible residues of intracellular components can be greatly reduced; furthermore, by adopting combined perfusion of Triton X-100 and Type I DNase I, not only are the nuclear components fully eluted, but the biolo