METHODS AND SYSTEMS FOR PREDICTING THE RISK OF TRANSGENE SILENCING

The present invention is based on the use of the H3K9me2 methylation levels in plant genomes to predict transgene silencing, transgene stability, and/or transgene expression level. Provided are methods and/or systems for generating whole-genome H3K9me2 maps and its use with an assigned threshold val...

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Bibliographische Detailangaben
Hauptverfasser:	WANG POHAO, SRIRAM SHREEDHARAN, SASTRY-DENT LAKSHMI
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	AGRICULTURE ANIMAL HUSBANDRY BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FISHING FORESTRY HUMAN NECESSITIES HUNTING INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTEDFOR SPECIFIC APPLICATION FIELDS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NEW PLANTS OR PROCESSES FOR OBTAINING THEM PHYSICS PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TRAPPING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention is based on the use of the H3K9me2 methylation levels in plant genomes to predict transgene silencing, transgene stability, and/or transgene expression level. Provided are methods and/or systems for generating whole-genome H3K9me2 maps and its use with an assigned threshold value for predicting gene silencing. The methods and/or systems provided herein can be used in high-throughput setting for screening large number of transformed event in a relatively short period of time as compared to existing technologies. 本发明基于使用植物基因组中的H3K9me2甲基化水平来预测转基因沉默、转基因稳定性和/或转基因表达水平。提供了用于生成全基因组H3K9me2图谱的方法和/或系统及其与指定阈值起用于预测基因沉默的用途。本文提供的所述方法和/或系统可用于高通量设置中，用以在与现有技术相比相对短的时间段内筛选大量转化事件。