Human normal conjunctival epithelial cell and application thereof

The invention discloses a human normal conjunctival epithelial cell and an application thereof. The cell is named human normal conjunctival epithelial cell HNCEC/HL-043, and the preservation number isCCTCC NO:C201676. A method for primary separation and cultivation is as follows: a pterygium operation patient eyeball bitamporal normal conjunctival tissue sample is digested, dispase and DNase I are added for action, filtering and centrifugation are carried out for collecting cells, an HL medium is used for resuspending cells, and inoculation and cultivation are carried out. A subcultring method is as follows: when cells are proliferated to an abundance of 70-90%, a pancreatin-EDTA is used for digestion, and DMEM is used for neutralization; cells are centrifuged and collected, an HL mediumis used for resuspending cells, and inoculation and cultivation are carried out. The cells are used for physiological researches of human normal cells, and researches of pathogenesis of conjunctiva related diseases, such as xe