Peripheral blood erythrocyte lysis method in tree shrew cellular immunity phenotypic analysis

The invention relates to a tree shrew peripheral blood (PB) erythrocyte lysis method. According to the tree shrew peripheral blood (PB) erythrocyte lysis method, BD Pharm Lyse is adopted for two times of lysis of tree shrew whole blood, and optimization on lysis conditions including lysis time, lysis times, and the volume ratio of the lysate to whole blood is carried out, an optimized erythrocyte lysis scheme suitable for tree shrew peripheral blood erythrocyte immunity phenotypic analysis is established, and an important technology platform is provided for further study on the evaluationon immunity system and immune response of tree shrew as an experimental animal. 本发明涉及树鼩外周血(PB)红细胞裂解方法。其通过 BD Pharm Lyse两次裂解树鼩全血，并对裂解条件即裂解时间、裂解次数以及裂解液与全血的体积比进行了优化，建立了优化的用于树鼩外周血细胞免疫表型分析的红细胞裂解方案，为进步研究树鼩实验动物的免疫系统和免疫应答的评估提供了重要的技术平台。