Method for preparing konjac oligosaccharide by carrying out enzymatic hydrolysis on wet konjac raw material

The invention provides a method for preparing konjac oligosaccharide by carrying out enzymatic hydrolysis on a wet konjac raw material, which comprises: (1) preparing neutral beta-mannase solution; (2) preparing fresh konjac pulp; (3) carrying out hydrolysis on konjac glucomannan; (4) filtering hydrolysis solution of the konjac oligosaccharide; (5) removing alkaloid; (6) carrying out crude separation of the konjac oligosaccharide; (7) purifying the konjac oligosaccharide; (8) carrying out centrifugal spray drying. According to the invention, fresh konjac tubers are directly processed; processing time is short; a formed oligosaccharide product is clear in color, low in viscosity and low in production cost. 本发明提供了种酶法水解湿魔芋原料制备魔芋寡糖的方法，包括：(1)制备中性β-甘露聚糖酶溶液；(2)制备新鲜魔芋浆；(3)水解魔芋葡甘露聚糖；(4)过滤魔芋寡糖水解溶液；(5)去除生物碱；(6)粗分离魔芋寡糖；(7)纯化魔芋寡糖；(8)离心喷雾干燥。本发明直接利用新鲜魔芋块茎进行加工，加工时间短，形成的寡糖产品颜色清亮，黏度低，生产成本低。