Improved light-emitting molecules

Disclosed are luciferase polypeptides with improved light-emitting activity and their encoding nucleic acids. These molecules are useful in a range of assays including luciferase-based gene reporter assays, bioluminescence resonance energy transfer assays, protein complementation assays and other applications in which luciferase enzymes are utilized as detectable and/or quantifiable labels. Also disclosed are methods and compositions for increasing the sensitivity and/or improving the kinetics of luciferase-catalyzed reactions as well as decreasing the impact of undesirable variables. 本发明公开的是具有改善的光-发射活性的萤光素酶多肽和它们的编码核酸。这些分子有用于系列测定包括基于萤光素酶的基因报告子测定，生物发光共振能量转移测定，蛋白互补测定和将萤光素酶用作可检测的和/或可定量的标记物的其他应用。也公开的是增加萤光素酶-催化的反应的灵敏度和/或改善萤光素酶-催化的反应的动力学以及降低不期望的变量的影响的方法和组合物。