Breeding method of radish cell nucleo-cytoplasmic interaction male sterile maintainer line

The invention discloses a breeding method of a radish cell nucleo-cytoplasmic interaction male sterile maintainer line. According to the method, a maintainer line Goutouguan B of the radish cytoplastmale sterile maintainer line Goutouguan is used to be hybridized with fertile plant Shizhuangbai B in a Shizhuangbai two-type line after the castration; the hybridized first generation F1 group is obtained; sterile plant Shizhuangbai A in the Shizhuangbai two-type line is used as a female parent, and the hybridized first generation F1 group is used as a male parent for test crossing; meanwhile, the hybridized first generation F1 group is subjected to self crossing; the expression condition of the fertility of the test crossing later generations is observed; the later generations sterile test crossing combination is screened; the male parents of the later generations sterile test crossing combination are subjected to self crossing and are then subjected to back crossing with the Shizhuangbai B; through the test cro