Genes and proteins for prosthetic group-free chloroperoxidase CPO822 and application of genes and proteins

The invention discloses genes and proteins for prosthetic group-free chloroperoxidase CPO822 with multiple functions, particularly capability of degrading benzopyrene and having activities of peroxidase, catalase, halogenase and esterase, and application of the genes and proteins. The invention has the beneficial effects that by virtue of a metagenome technology, a novel DNA sequence without functional study is obtained from soil metagenome; study results of the structures and functions of proteins coded by the DNA sequence show that a recombinant protein expressed by the DNA sequence has polyfunctionality, has a wider catalytic reaction range than existing separated peroxidase, can serve as a novel enzyme source to be applied to the fields such as washing, wastewater treatment, disinfection, lignin removal, production of epoxy derivatives, degradation of benzopyrene and the like, and has wide industrial application prospects. In addition, the study discovers that the enzyme is stableunder a wide-range pH val