High-density culture method of prorocentrum

The invention discloses a high-density culture method of prorocentrum. The high-density culture method comprises the following steps: (a) in an aseptic manipulation room, inoculating prorocentrum seedliquid into a jacketed glass reactor with the volume of 10 to 50 L filled with a sterile f2 culture medium, wherein the salinity of the f2 culture medium is 20 to 28; (b) transferring to a routine test laboratory, introducing sterilized air, controlling the light intensity at 3000 to 5000lux and light/dark cycle at 14h/10h, controlling circulating water temperature of a jacket at 22 to 25 DEG C and culturing for 10 to 20 days; (c) in the aseptic manipulation room, discharging most cultured algae liquid from a discharge opening of a reaction still, adding a fresh sterilized f2 culture medium into the reaction still, transferring to the routine test laboratory and continuously carrying out next culture cycle by using identical conditions in step b. The high-density culture method disclosedby the invention has the a