Repeated low dose radiation lewis tumor-bearing mouse model building method

The invention discloses a repeated low dose radiation lewis tumor-bearing mouse model building method, and belongs to the field of laboratory animal science. The method mainly includes the steps: injecting conventional in-vitro cultured lewis cells into a mouse r-shoulderblade subepithelial portion by a subcutaneous vaccination method; feeding a mouse in a room-temperature environment, peeling tumor tissues when a tumor grows to reach the size of 2*2cm, cutting the tumor tissues into uniform small pieces, weighing 0.05g of small tumor tissues, implanting the small tumor tissues into armpits ofthe mouse, sewing the armpits, feeding the mouse and measuring the size of the tumor one week after. Compared with a conventional tumor-bearing mouse model building method, the method has the advantages that equal sizes of tumors implanted into an intra-group C57 mouse are ensured, models are uniform, and low dose radiation research needs are met. 本发明公开了多次低剂量辐射用lewis荷瘤小鼠模型的建立方法，属于实验动物学领域，其主要步骤包括：将常规体外培养的lewis细胞采用皮下接种法注射入