Preparation and application of peroxide nitroso (ONOO<->) fluorescent probe based on hemicyanine dye

The invention relates to preparation and application of a peroxide nitroso (ONOO) fluorescent probe based on a hemicyanine dye. A structural formula of the fluorescent probe is shown in the attached figure. The invention provides a preparation method of the fluorescent probe which is synthesized by adopting IR-780, 3-nitrophenol, stannous chloride and the like as raw materials. The fluorescentprobe is characterized in that the fluorescent probe is a ratio peroxide nitroso fluorescent probe with near-infrared effect and high selectivity; firstly, the fluorescent probe has higher sensitivityon ONOO, and the specific value of fluorescence intensity (F460/F708) is enhanced by 153 times; then, the fluorescent probe has higher selectivity on ONOO, and is free from the interference byother active oxygen, active nitrogen, active sulfur and biological mercaptan; the fluorescent probe can quickly act with ONOO, and the response time is controlled within 80s; the fluorescent probehas been successfully applie