Beta-agonist hapten, artificial antibody as well as preparation methods and applications of beta-agonist hapten and beta-agonist artificial antibody

The invention discloses a beta-agonist hapten. The molecular structure of the beta-agonist hapten is shown in the following formula (I) in the description. The beta-agonist hapten is successfully synthesized from R-(-)-salbutamol as a raw material by one-step chemical reaction. The hapten is coupled with carrier protein, an artificial antigen is prepared, and the broad-spectrum specific beta-agonist artificial antibody is obtained after immunization. The antibody can recognize 31 kinds of beta-agonist drugs and analogs of the beta-agonist drugs, the semi-inhibitory concentration for R-(-)-salbutamol is 0.5 ng/mL, the linear range is 0.11-40.86 ng/mL, and the minimum detection limit is 0.04 ng/mL. The antibody can detect multiple beta-agonist drugs simultaneously, can be used for on-site rapid detection of food safety and has broad application prospect. 本发明公开了种β-激动剂半抗原，其分子结构如下式（I）所示：。本发明以R-(-)-沙丁胺醇为原料，经步化学反应成功合成了β-激动剂半抗原。再将该半抗原与载体蛋白偶联制备人工抗原，经免疫后得到广谱特异性β-激动剂人工抗体。该抗体可以识别31种β-激动剂药物及其类似物，其中对R-(-)-沙丁胺醇的半抑制浓度为0.5ng/