Nucleic acid composition for detecting HPVs (Human Papilloma Viruses) as well as application and kit of nucleic acid composition

The invention discloses a nucleic acid composition for detecting HPVs (Human Papilloma Viruses) as well as application and a kit of the nucleic acid composition, and relates to the technical field ofin-vitro diagnosis of virus nucleic acid detection. The nucleic acid composition for detecting the HPV comprises primers which are shown in SEQ ID NO.1 to 6 and primers which are shown in SEQ ID NO.7to 9. The nucleic acid composition disclosed by the invention is capable of simultaneously detecting HPV 16 and HPV 18 and parting the specificity of the HPV 16 and the HPV 18, meanwhile, quality control can also be carried out on a detecting process through an internal control beta-globin gene detection result, false negatives can be reduced, and the nucleic acid composition has the characteristics of high sensitivity and strong specificity. 本发明公开了种检测人乳头瘤病毒的核酸组合及其应用和试剂盒，涉及病毒核酸检测的体外诊断技术领域。本发明公开的检测人乳头瘤病毒的核酸组合包括：SEQ ID NO.1-6所示的引物和SEQ ID NO.7-9所示的探针。该核酸组合可同时检测HPV 16和HPV 18且可对HPV16和HPV18特异性分型，同时还可通过内对照β-globin基因检测结果对检测过程进