Ultra-fast real-time nucleic acid detection method for Streptococcus pneumoniae

The invention relates to an ultra-fast real-time nucleic acid detection method for Streptococcus pneumoniae. According to the ultra-fast real-time nucleic acid detection method, nucleic acid is extract, amplification is performed with fluorescent labeled primers in a special buffer solution in a disk-type reaction container under the catalysis of an enzyme, and the nucleic acid template content ina sample is real-timely and quantitatively detected, wherein the reaction time is substantially shortened compared to the previous fluorescence quantitative nucleic acid detection method through thespecial buffer solution and the disk-type reaction container, and is shortened to 5-10 min from the original time of more than 1 h, such that the rapid detection of Streptococcus pneumoniae infectionis truly achieved. According to the present invention, the ultra-fast real-time nucleic acid detection method has advantages of short reaction time, simple operation, high specificity and high promotion value, and is suitable f