Leslie new pteris fern sprout tissue culture rapid propagation method

The invention discloses a lesile new pteris fern sprout tissue culture rapid propagation method which has the advantages of quick propagation speed, low cost and ability in keeping neat and even furcation on top ends of blades of lesile new pteris fern. The leslie new pteris fern sprout tissue culture rapid propagation method disclosed by the invention comprises the following steps: (1) taking leslie new pteris fern rhizome as an implant to perform sterile treatment and differentiating small-particle protocorms at the incision position; (2) taking the protocorms, putting into an MS culture medium to be cultured to make each protocorm differentiate 3 to 4 new protocorms and performing multiplication culture on sterile seedlings; (3) putting the protocorms in the MS culture medium to be cultured to make each protocorm grow 1 to 3 seedlings, putting all the growing seedlings into another MS culture medium to be culture, namely performing transplantation and domestication, cleaning culturesolution at the roots of