Method for purifying recombinant anti-TNF-alpha completely-human monoclonal antibody

The invention discloses a method for purifying a recombinant anti-TNF-alpha completely-human monoclonal antibody. The method comprises a step of performing cation exchange chromatographic elution on the antibody. The cation exchange chromatographic elution comprises the following concrete procedures: after balanced sample loading, carrying out primary elution by using an equilibration buffer 1 atfirst; then carrying out secondary elution by using an equilibration buffer 2; carrying out ternary elution by using a mixture of the equilibration buffer 2 and an equilibration buffer 3; and finallycarrying out elution to obtain a purified antibody, wherein the equilibration buffer 1 is a phosphate buffer or acetate buffer; the equilibration buffer 2 is a phosphate buffer or acetate buffer; andequilibration buffer 3 is a phosphate buffer or acetate buffer. The method of the invention reduces the amount of acidic components in the recombinant anti-TNF-alpha completely-human monoclonal antibody and improves the purity