Efficient mutation breeding method of corn

The invention belongs to the technical field of corn breeding, and specifically relates to an efficient mutation breeding method of corn. The efficient mutation breeding method comprises the followingsteps: pollen collection, pollen mutation treatment, artificial pollination and seed mutation, wherein after medicine is removed from collected pollen, the pollen is added into an EMS-paroline solution, and mutation is carried out according to the following steps: (1) carrying out laser radiation for 2 to 4 min; (2) carrying out light-shading stirring for 20 to 30 min; (3) carrying out laser radiation for 2 to 4 min; and (4) carrying out light-shading stirring for 15 to 20 min, wherein before artificial pollination, the EMS-paroline solution is smeared on the female ear filaments of corn plants to be pollinated so as to obtain mutated filaments, then carrying out artificial pollination, planting harvested corn seeds after x ray radiation so as to obtain mutated corn plants. Three mutationmanners of laser, EMS and