Method for fermenting and producing recombinant glucagon-like peptide-1 protein

The invention discloses a method for fermenting and producing recombinant glucagon-like peptide-1 (GLP-1) protein. The method is characterized by comprising the following steps: culturing geneticallyengineered bacteria containing a nucleic acid sequence of encoded recombinant glucagon-like peptide-1...

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Bibliographische Detailangaben
Hauptverfasser:	YU LI, LI JINGJING, LI DONGSHENG, ZHU JIANBIN, WANG MINGZHU, LI HAO, SHE MIAOQIN, WANG JINHUI, CAI XIANGSHENG
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses a method for fermenting and producing recombinant glucagon-like peptide-1 (GLP-1) protein. The method is characterized by comprising the following steps: culturing geneticallyengineered bacteria containing a nucleic acid sequence of encoded recombinant glucagon-like peptide-1 protein by utilizing a low-phosphate culture medium; controlling the pH (Potential of Hydrogen) value and dissolved oxygen of a culture phase; after finishing fermentation, centrifuging to remove thalli; purifying supernatant to obtain the recombinant glucagon-like peptide-1 protein. The method disclosed by the invention can secrete expressed GLP-1 in a high-purity and stable manner and a separation and purification technology is simplified. 本发明公开了种发酵生产重组胰高血糖素样肽-1蛋白的方法，其特征在于，包括下列步骤：将含有编码重组胰高血糖素样肽-1蛋白的核酸序列的基因工程菌用低磷酸盐培养基进行培养，控制培养阶段pH值和溶氧量，发酵结束后，离心去除菌体，经过纯化上清液得到所述重组胰高血糖素样肽-1蛋白。本发明的方法能够高纯度、稳定分泌表达的GLP-1，简化分离纯化工艺。