Human mycoplasma pneumoniae isolation culture medium and culture method

The invention relates to the field of mycoplasma isolation culture and in particular relates to a human mycoplasma pneumoniae isolation culture medium SLB and a culture method of the culture medium. The culture medium is prepared from a basic culture medium, horse serum, antibiotics and other adding ingredients, and is prepared from the following components: 560ml of the basic culture medium, 50 to 200ml of the horse serum, 4ml of the antibiotics and 386 to 236ml of the other adding ingredients; the total volume of the added horse serum and other adding ingredients is 436ml; the final pH (Potential of Hydrogen) value is adjusted to be 7.4 to 7.6 by utilizing 1M of NaOH. The antibiotics are prepared from penicillin G, thallium acetate, polymyxin B and amphotericin. The other additive ingredients are prepared from a CMRL 1066 tissue culture supplementing ingredient containing glutamine, glucose and yeast coldly-soaked powder. The culture medium provided by the invention has good selectivity, strong specificity,