Method for purifying ulinastatin based on affinity chromatography column

The invention discloses a method for purifying ulinastatin through combining urine pretreatment with an affinity chromatography column. The method comprises the following steps: carrying out refrigeration, vacuum and microwave pretreatment on urine to obtain crude ulinastatin, performing hydrophobic column chromatography, affinity chromatography and gel filtering chromatography on the crude ulinastatin to purify the ulinastatin, and washing and freeze-drying the purified ulinastatin to obtain ulinastatin with the total yield being 90% or more and the total tilter being 6700 i[mu]/mg or more. The pretreatment of the urine to a certain degree is combined with the chromatography column purifying treatment, so the method is simple to operate; and a filler, a flow velocity and other parametersare preferably selected, so the production efficiency is improved, the production cost is greatly reduced, and the method is suitable for being further developed and utilized. 本发明公开了种对尿液进行预处理并结合亲和层析柱对乌司他丁进行纯化方法，该方法包括以下步骤：对尿液进