Separation and purification method for recombinant hepatitis B core antigen

The invention relates to a separation and purification method for a recombinant hepatitis B core antigen. The method includes the steps of thermal denaturation and clarification; ammonium sulfate precipitation; ultrafiltration and concentration, washing filtering and liquid exchange; depolymerization; first-step molecular sieve chromatography; ultrafiltration and concentration, washing filtering and liquid exchange; repolymerization; second-step chromatography. By means of the method, the high-purity, low-host-residue and high-stability recombinant hepatitis B core antigen with a uniform granular structure can be obtained, that is to say, the method has the advantages that the antigen purity is high, the residue of host protein and host nucleic acid is low, antigen particles are uniform, and industrial enlargement is easy. 本发明涉及种重组乙肝核心抗原的分离纯化方法，该方法包含热变性澄清、硫酸铵沉淀、超滤浓缩和洗滤换液、解聚合、第步分子筛层析、超滤浓缩、洗滤和换液、复聚和第二步分子筛层析等步骤。该方法可获得的高纯度、低宿主残留、颗粒结构均、高度稳定的重组乙肝核心抗原，即该方法具有抗原纯度高、宿主蛋白及宿主核酸残留低、抗原颗粒均且易于工业化放大等特点。