Compound amplification kit for simultaneously detecting female skin and hair SNP (Single Nucleotide Polymorphism) genetic polymorphism site and application thereof

The invention discloses a compound amplification kit for simultaneously detecting a female skin and hair SNP (Single Nucleotide Polymorphism) genetic polymorphism site and application thereof. The kitcomprises primers of 6 SNP site genetic loci; the 6 SNP site genetic loci comprise: MC1R (rs1805007)...

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Hauptverfasser: CHEN LINLI, WANG PEIPEI, SHAO ZEXIANG, XIA ZIFANG, SHEN HUQIN, LU WENXIANG, GUO YULIN, ZHENG WEIGUO
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses a compound amplification kit for simultaneously detecting a female skin and hair SNP (Single Nucleotide Polymorphism) genetic polymorphism site and application thereof. The kitcomprises primers of 6 SNP site genetic loci; the 6 SNP site genetic loci comprise: MC1R (rs1805007), NQO1 (rs1800566), AQP3 (rs17553719), FGFR2 (rs4752566), EDAR (rs3827760) and MMP1 (rs1799750); anrs number in brackets represents the number of the site in a dbSNP database. According to the kit disclosed by the invention, the primers are optimized so that the recognition capability of the primers to SNP is improved and the primers can be used for effectively identifying the difference of one basic group; the most suitable annealing temperature range is enlarged and a condition that a detection result has false positive, caused by the fact that annealing temperature is required to be accurate, is avoided, and the detection accuracy and reliability are improved; an ARMS (Amplification Refractory Mutation System) t